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  • Title: Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex.
    Author: Leung KH, He HZ, Wang W, Zhong HJ, Chan DS, Leung CH, Ma DL.
    Journal: ACS Appl Mater Interfaces; 2013 Dec 11; 5(23):12249-53. PubMed ID: 24245499.
    Abstract:
    We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy)2(bcp)](+) (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes.
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