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  • Title: Mimicking hemoproteins: a new synthetic metalloenzyme based on a Fe(III)-mesoporphyrin functionalized by two helical decapeptides.
    Author: Venanzi M, Cianfanelli S, Palleschi A.
    Journal: J Pept Sci; 2014 Jan; 20(1):36-45. PubMed ID: 24254807.
    Abstract:
    A new metalloenzyme formed by a Fe(III)-mesoporphyrin IX functionalized by two helical decapeptides was synthesized to mimic function and structural features of a hemoprotein active site. Each decapeptide comprises six 2-aminoisobutyric acid residues, which constrain the peptide to attain a helical conformation, and three glutamic residues for improving the solubility of the catalyst in aqueous solutions. The new compound shows a marked amphiphilic character, featuring a polar outer surface and a hydrophobic inner cavity that hosts the reactants in a restrained environment where catalysis may occur. The catalytic activity of this synthetic mini-protein was tested with respect to the oxidation of L- and D-Dopa by hydrogen peroxide, showing moderate stereoselectivity. Structural information on the new catalyst and its adduct with the L- or D-Dopa substrate were obtained by the combined use of spectroscopic techniques and molecular mechanics calculations.
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