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  • Title: Production and partial characterization of anti-Candida monoclonal antibodies.
    Author: Chardès T, Piechaczyk M, Cavaillès V, Salhi SL, Pau B, Bastide JM.
    Journal: Ann Inst Pasteur Immunol (1985); 1986; 137C(2):117-25. PubMed ID: 2425714.
    Abstract:
    Spleen cells of Biozzi HR mice immunized with formolized, lyophilized Candida albicans serotype A cells were fused with P3-X63-Ag8.653 mouse myeloma cells. Twenty-one monoclonal antibodies (mAb) selected by an indirect ELISA technique were produced and partially characterized. All mAb reacted with a C. albicans cell wall extract. Five of the mAb were directed against C. albicans serotype A, but not against serotype B. These mAb also recognized C. tropicalis. The 16 other mAb cross-reacted with several yeast species. The immunoreactivity profiles of 5 representative anti-Candida mAb were confirmed in most cases by inhibition studies.
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