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  • Title: Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935.
    Author: Woo JB, Kang HN, Woo EJ, Lee SB.
    Journal: Food Chem; 2014 Apr 01; 148():184-7. PubMed ID: 24262544.
    Abstract:
    A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichiacoli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 °C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.
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