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  • Title: Specific activity staining for prostaglandin metabolizing enzymes on polyacrylamide gel.
    Author: Tanaka T, Mori N.
    Journal: Prostaglandins Leukot Med; 1986 Aug; 23(2-3):267-75. PubMed ID: 2429333.
    Abstract:
    A simple activity staining technique on polyacrylamide gel for 15-hydroxyprostaglandin metabolizing enzymes was developed in which production or digestion of 15-keto-PGE was stained. The rapidity and specificity of this staining was shown with a preparation of NAD-linked 15-hydroxyprostaglandin dehydrogenase isolated from human term placenta.
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