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  • Title: [Immunological study of antigenic determinants in the isolated hemagglutinin of the influenza A virus (H1N1)].
    Author: Isaeva EI, Ignat'eva TV, Rovnova ZI.
    Journal: Vopr Virusol; 1986; 31(3):283-8. PubMed ID: 2429447.
    Abstract:
    A preparation of isolated subunits of influenza A (H1N1) virus hemagglutinin was examined for antigenic properties by four serological tests: radioimmunoassay, radial immunodiffusion test, complement fixation test, and hemagglutination inhibition test with hyperimmune polyclonal serum to intact virion as well as with monospecific antibodies to individual antigenic determinants obtained by adsorption technique. In isolated hemagglutinin subunits, radioimmunoassay identified three main virus-specific antigenic sites identical to antigenic determinants in the hemagglutinin of the intact virion. No neuraminidase was found in the hemagglutinin preparation but increased serological activity of host cell antigens was observed.
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