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  • Title: Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus.
    Author: Zhu Y, Li Q, Mao G, Zou Y, Feng W, Zheng D, Wang W, Zhou L, Zhang T, Yang J, Yang L, Wu X.
    Journal: Carbohydr Polym; 2014 Jan 30; 101():606-13. PubMed ID: 24299817.
    Abstract:
    The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03°C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.
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