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  • Title: [Genetic polymorphisms of 15 X-STR loci in Shandong Han population].
    Author: Ma T, Xu J, Yang YJ, Sun K, Xue FZ, Jin L, Li SL.
    Journal: Fa Yi Xue Za Zhi; 2013 Jun; 29(3):202-5, 208. PubMed ID: 24303765.
    Abstract:
    OBJECTIVE: To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han population in order to establish the forensic application database. METHODS: The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins (FAM, VIC, NED and TET). The developed multi-PCR was used to investigate 15 X-STR loci (DXS10011, DXS101, GATA 165B12, DXS6795, DXS6800, DXS6801, DXS6803, DXS7132, DXS7133, DXS7423, DXS7424, DXS8377, DXS8378, DXS9898 and HPRTB) selected from the X chromosome of 481 unrelated individuals (295 females and 186 males) in Shandong Han population. RESULTS: Among the 15 X-STR loci, GATA 165B12, DXS6800, DXS6803, DXS7133 and DXS7423 showed moderate polymorphisms, while the rest 10 X-STR loci showed high polymorphisms (PIC > 0.5 and H > 0.5). No shared haplotype was detected among the males in Shandong Han population. CONCLUSION: The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Han population and shows its forensic application.
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