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  • Title: [Enzymatic synthesis of polyprenyl phosphosugars in Salmonella serotype C1].
    Author: Druzhinina TN, Rozal'eva VV, Shibaev VN, Rozhnova SSh, Kilesso VA.
    Journal: Bioorg Khim; 1986 Sep; 12(9):1234-9. PubMed ID: 2430582.
    Abstract:
    The cell envelopes of serogroup C1 Salmonella, viz. S. thompson and S. montevideo, catalyze the transfer of radiolabeled sugars from UDP-[14C]Glc and UDP-[14C]GlcNAc into the lipid-linked sugars. Using TLC and DEAE-cellulose chromatography, the radiolabeled products were identified as polyprenyl pyrophosphate N-acetylglucosamine (I), polyprenyl monophosphate N-acetylglucosamine and polyprenyl monophosphate glucose. The derivative (I) served as an acceptor for mannose transfer from GDP-Man with formation of Man1-2GlcNAc1PPPre. A similar reaction was observed after addition of synthetic GlcNAc1PPPre to the cell envelopes.
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