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  • Title: Quantitation of chick tissue zinc-metallothionein by gel electrophoresis and silver stain enhancement.
    Author: Lin LY, McCormick CC.
    Journal: Comp Biochem Physiol C Comp Pharmacol Toxicol; 1986; 85(1):75-84. PubMed ID: 2430754.
    Abstract:
    Discontinuous gradient gel electrophoretic systems were developed to quantitate zinc-metallothionein (Zn-MT) in chick tissues, liver and pancreas. Gels were stained with Coomassie Blue initially, then enhanced by silver stain. At least 4 micrograms of Zn-MT could be detected after Coomassie Blue stain, and 1 microgram Zn-MT detected following silver stain enhancement. Significant linearity (correlation coefficient = 0.99) of a standard curve was established in the Coomassie Blue stained gels. The results of our experiment suggest that electrophoretic analysis is a simple and feasible method for the quantitation and identification of Zn-MT in chick tissues.
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