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  • Title: Determination of soluble antigens of Clostridium botulinum A by chemiluminescent--immunosorbent assay (CLISA).
    Author: Ligieza J, Michalik M, Reiss J, Grzybowski J.
    Journal: Arch Immunol Ther Exp (Warsz); 1986; 34(2):189-95. PubMed ID: 2431667.
    Abstract:
    The chemiluminescent--immunosorbent assay (CLISA) was adopted for Cl. botulinum A soluble toxic antigens determination. Luminol (ABEI) labelled botulinum antitoxin globulins showed a strongly positive specific immunochemiluminescent reactions with the native botulinum toxin preparations coupled (adsorbed) on polystyrene balls. The sensitivity of the reaction reached 20 DLM/ml (5,000 light impulses per 40 sec) in comparison with 2 X 10(6) DLM (57,000 impulses), control preparations (1,500 impulses) and the background (150-300 impulses). The results give a perspective further investigations with the CLISA for rapid indication of Cl. botulinum toxic antigens.
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