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  • Title: [Suppressive effects of gamma-secretase inhibitor DAPT on the proliferation of pancreatic cancer cells].
    Author: Du X, Cheng Z, Li Y, Zhou ZG, Yang L, Zhang MM.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2013 Sep; 44(5):699-702. PubMed ID: 24325093.
    Abstract:
    OBJECTIVE: To explore the effect of DAPT, a gamma-secretase inhibitor on the proliferation of pancreatic cancer cells and its underlying mechanism. METHODS: Three human pancreatic cancer cell lines as AsPC-1, BxPC-3 and MIAPaCa-2 were employed for in vitro experiments. Cell proliferation was tested by CCK-8 and cell apoptosis was examined by Annexin V/PI staining after the treatment of various concentrations of DAPT at different time. RESULTS: DAPT of 100 micromol/L significantly inhibited the proliferation of these 3 cell lines from day 3 to 5. DAPT of 50 micromol/L inhibited the proliferation of BxPC-3 and MIAPaCa-2 cells from day 3 to 5, whereas the effect of inhibition was only observed at day 5 in AsPC-I cell. DAPT of 10 micromol/L inhibited the proliferation of BxPC-3 and MIAPaCa-2 cells at day 5, whereas no effect was found in AsPC-1. Flow cytometry test showed that cell apoptosis rate was higher in DAPT treatment groups compared with control group. CONCLUSION: DAPT could suppress the proliferation of pancreatic cancer cell, with the mechanism of promoting cell apoptosis.
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