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  • Title: Aptamer-based biosensor for detection of phenylalanine at physiological pH.
    Author: Omidinia E, Shadjou N, Hasanzadeh M.
    Journal: Appl Biochem Biotechnol; 2014 Feb; 172(4):2070-80. PubMed ID: 24326680.
    Abstract:
    A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptasensor for determination of phenylalanine in aqueous solutions. This sensor was tested in a Tris-HCl buffer with physiological pH = 7.4 by cyclic voltammetry and differential pulse voltammetry. The detection limit and sensitivity of the modified electrode toward phenylalanine were estimated to be 1 nM (S/N = 3) and 0.367 μA nM(-1), respectively. The linear range of the signal was observed between 1 and 10 nM of phenylalanine with 0.9914 correlation factor. The herein-described approach is expected to promote the exploitation of aptamer-based biosensors for protein assays in biochemical and biomedical studies.
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