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  • Title: [Detection by thermal denaturation of damages to the Na pump in the myocardial sarcolemma in stress and the role of lipid peroxidation in this process].
    Author: Sazontova TG.
    Journal: Biull Eksp Biol Med; 1986 Dec; 102(12):685-7. PubMed ID: 2432959.
    Abstract:
    The determination of ATP-hydrolytic activity of Na pump does not always reveal the enzyme damage in vivo. The method assessing Na, K-ATPase molecular conformational stability in the rat heart sarcolemma based on thermal denaturation is suggested. After a prolonged emotional-painful stress (EPS) the activity of Na, K-ATPase dropped by 20%, as the rate of its thermal denaturation in the range of 50-60 degrees C increased 2-3-fold. Thermodynamic calculations have demonstrated a decrease in Ea, delta H and delta S* of Na, K-ATPase thermal denaturation process after EPS. An analogous enzyme damage was found after the activation of lipid peroxidation in sarcolemma membrane suspension. These results imply that essential changes in intra- and supra-molecular properties of Na, K-ATPase under EPS may be detected by thermal denaturation. Lipid peroxidation is a most likely reason for EPS-induced Na pump damage.
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