These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Insulin and glyburide increase cytosolic free-Ca2+ concentration in isolated rat adipocytes.
    Author: Draznin B, Kao M, Sussman KE.
    Journal: Diabetes; 1987 Feb; 36(2):174-8. PubMed ID: 2433175.
    Abstract:
    We investigated the effect of insulin and a hypoglycemic sulfonylurea agent glyburide on cytosolic free-Ca2+ concentrations [( Ca2+]i) in isolated rat adipocytes. Both insulin and glyburide increased [Ca2+]i in a dose-dependent manner. Half-maximal effects were seen at 0.5 ng/ml of insulin and 0.5 microM glyburide. Nifedipine (25 microM), a Ca2+-channel blocker, inhibited the effect of both agents. The effect of insulin on [Ca2+]i was 40 and 70% potentiated by ambient glucose concentrations at 180 and 300 mg/dl, respectively. Depolarizing doses of potassium (40 mM) induced an increase in cytosolic Ca2+ that was also inhibited by nifedipine. It is suggested that both insulin and glyburide increase cytosolic free Ca2+ levels at least in part by promoting Ca2+ influx through voltage-dependent Ca2+ channels.
    [Abstract] [Full Text] [Related] [New Search]