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  • Title: Purification of a soluble phospholipase A2 from synovial fluid in rheumatoid arthritis.
    Author: Stefanski E, Pruzanski W, Sternby B, Vadas P.
    Journal: J Biochem; 1986 Nov; 100(5):1297-303. PubMed ID: 2434471.
    Abstract:
    A soluble phospholipase A2 (PLA2) was purified 4,500-fold from human rheumatoid synovial fluid. Preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis yielded two bands of PLA2 activity of molecular weights 15,000 and 17,000 and pl 4.2-5.0. Purified PLA2 had absolute 2-acyl specificity, and hydrolyzed phosphatidylcholine with optimal activity at pH 7.5-8.0 and phosphatidylethanolamine with optimal activity at pH 7.0. Human synovial fluid PLA2 did not cross-react with anti-human pancreatic PLA2, as tested by radioimmunoassay.
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