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  • Title: Sequential G- to R-banding for high resolution chromosome analysis.
    Author: Hirsch B, Mack R, Arthur D.
    Journal: Hum Genet; 1987 May; 76(1):37-9. PubMed ID: 2437009.
    Abstract:
    A sequential staining protocol for revealing first G- and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.
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