These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: Inhibition by cytochalasin B of DNA synthesis in a thermosensitive anchorage-independent growth mutant. Author: Miyashita K. Journal: J Cell Physiol; 1987 Apr; 131(1):99-106. PubMed ID: 2437133. Abstract: After a shift from a nonpermissive to a permissive temperature, synchronized DNA synthesis and cell division were observed in a cold-sensitive anchorage-independent growth mutant (cs-17-25) of Chinese hamster lung cells in Methocel culture. Only 15 min exposure to the permissive temperature was sufficient for induction of DNA synthesis in the cells. A low dose of actinomycin D (0.02 micrograms/ml) or cytochalasin B (5 micrograms/ml) was able to inhibit the DNA synthesis when added at an early period after the temperature shift. The inhibitory effects of actinomycin D and cytochalasin B on RNA and protein synthesis were very similar at both temperatures. The degree of multinucleation caused by cytochalasin B was altered quickly depending on the incubation temperature. These results suggest that stimulation of this mutant involves an obligatory actin-mediated step closely correlated to transcription of early mRNAs.[Abstract] [Full Text] [Related] [New Search]