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  • Title: Effects of nanoparticle size and cell type on high sensitivity cell detection using a localized surface plasmon resonance biosensor.
    Author: Liu F, Wong MM, Chiu SK, Lin H, Ho JC, Pang SW.
    Journal: Biosens Bioelectron; 2014 May 15; 55():141-8. PubMed ID: 24373953.
    Abstract:
    A localized surface plasmon resonance (LSPR) effect was used to distinguish cell concentration on ordered arrays of Au nanoparticles (NPs) on glass substrates. Human-derived retinal pigment epithelial RPE-1 cells with flatter bodies and higher confluency were compared with breast cancer MCF-7 cells. Nanosphere lithography was used to form Au NPs with average diameters of 500 and 60 nm in order to compare cell detection range, resonance peak shift, and cell concentration sensitivity. A larger cell concentration range was detected on the larger 500 nm Au NPs compared to 60 nm Au NPs (8.56 × 10(3)-1.09 × 10(6) vs. 3.43 × 10(4)-2.73 × 10(5)cells/ml). Resonance peak shift could distinguish RPE-1 from MCF-7 cells on both Au NPs. RPE-1 cells consistently displayed larger resonance peak shifts compared to MCF-7 cells until the detection became saturated at higher concentration. For both types of cells, higher concentration sensitivity in the range of ~10(4)-10(6)cells/ml was observed on 500 nm compared to 60 nm Au NPs. Our results show that cells on Au NPs can be detected in a large range and at low concentration. Optimal cell sensing can be achieved by altering the dimensions of Au NPs according to different cell characteristics and concentrations.
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