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  • Title: Identification of proteins by crossed immunoelectrophoresis with a trap-gel.
    Author: Laine A, Ducourouble MP, Hannothiaux MH.
    Journal: Anal Biochem; 1987 Feb 15; 161(1):39-44. PubMed ID: 2437826.
    Abstract:
    Simple improvements of the crossed immunoelectrophoresis method are described. A trap-gel was prepared with a small quantity of monospecific antiserum and was submitted to preelectrophoresis. It blocked, during the first dimension, an individual protein as a rocket. The corresponding peak was reduced or disappeared in the second dimension. Identification of precipitation peaks in human or Macacus cynomolgus bronchoalveolar lavage fluids is illustrated and unequivocal recognition of some antigens is shown.
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