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  • Title: A new transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 antibody.
    Author: Amakishi E, Hayashi T, Koh Y, Matsuyama N, Ishii H, Matsukura H, Yasui K, Hirayama F.
    Journal: Vox Sang; 2014 May; 106(4):368-71. PubMed ID: 24387678.
    Abstract:
    BACKGROUND AND OBJECTIVES: CD36 antibody (Ab) causes several disorders: neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non-haemolytic transfusion reactions. However, there is no gold-standard test for CD36 Ab. MATERIALS AND METHODS: We developed a transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 Ab and compared it with the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) system in terms of sensitivity and specificity. RESULTS: Our new system was characterized by (1) gene-transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD36 MoAbs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system. CONCLUSIONS: The present results indicate that our new system permits highly sensitive and specific detection of CD36 Ab.
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