These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effect of 5-azacytidine and congeners on DNA methylation and expression of deoxycytidine kinase in the human lymphoid cell lines CCRF/CEM/0 and CCRF/CEM/dCk-1.
    Author: Antonsson BE, Avramis VI, Nyce J, Holcenberg JS.
    Journal: Cancer Res; 1987 Jul 15; 47(14):3672-8. PubMed ID: 2439195.
    Abstract:
    The human lymphoid cell lines CCRF/CEM/0 and the deoxycytidine kinase (dCk)-deficient CCRF/CEM/dCk- were treated with various 5-azacytidine (5-aza-C) nucleosides and the effect on DNA methylation and dCk activity were examined. 5-Azacytidine (5-aza-C), 5,6-dihydro-5-azacytidine (DHAC), 5-aza-2'-deoxycytidine (5-aza-Cdr), and 1-beta-D-arabinofuranosyl-5-azacytidine (ara-AC) reduced the DNA 5-methylcytosine level in the CEM/0 cells, down to approximately 10% of the level in untreated cells. The dCk activity was increased after treatment with the 5-aza-C nucleosides approximately 10% compared to untreated cells. In CEM/dCk- cells DNA hypomethylation between 50 and 25% of control was seen only after treatment with DHAC and 5-aza-C. No decrease in the methylation level was seen after treatment with 5-aza-Cdr or ara-AC. The dCk activity was increased up to 37% after treatment with DHAC or 5-aza-C but no increase was observed after treatment with 5-aza-Cdr or ara-AC. CEM/dCk- cells treated with DHAC showed a revertant frequency to cells expressing dCk activity of between 0.1 and 0.6%. Cloned revertant CEM/dCk- cells isolated from soft agar had dCk activity between 31 and 113% compared to the activity in untreated CEM/0 cells. This in vitro study indicates that DHAC and 5-aza-C induced dCk re-expression in the CEM/dCk- cells whereas 5-aza-Cdr and ara-AC did not.
    [Abstract] [Full Text] [Related] [New Search]