These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells.
    Author: Chang M, Liu R, Jin Q, Liu Y, Wang X.
    Journal: Biotechnol Appl Biochem; 2014; 61(5):510-6. PubMed ID: 24397363.
    Abstract:
    Two scaffold/matrix attachment regions (S/MARs) from Chinese hamster ovary (CHO) cell chromosomes were identified computationally and cloned as the domain boundaries regions. As partition chromosomes in independently regulated units to increase the exogenous gene expression, these two S/MARs (MAR1 and MAR6) flanked expression vectors were respectively transfected into CHO cells. The results showed that MAR1 and MAR6 were much more potent to increase the stable transfection efficiency than the chicken lysozyme gene 5' S/MARs, which had been experimentally identified to increasing the expression of heterologous gene. Moreover, MAR6 is more active in mediating high and consistent gene expression in CHO cells than the chicken lysozyme gene 5' S/MARs.
    [Abstract] [Full Text] [Related] [New Search]