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  • Title: Antifibrotic effect of pirfenidone on human pterygium fibroblasts.
    Author: Lee K, Young Lee S, Park SY, Yang H.
    Journal: Curr Eye Res; 2014 Jul; 39(7):680-5. PubMed ID: 24400786.
    Abstract:
    OBJECTIVE: The effects of pirfenidone were investigated on cultured human pterygium fibroblasts (HPFs). METHODS: HPFs were obtained from pterygium surgery and subjected to primary culture. After treatment with 0.5, 1.0 or 1.5 mg/mL pirfenidone, MTT and cell migration assays were performed, and procollagen secretion and TGF-β expression were measured by Western blotting and immunofluorescence analysis. RESULTS: Pirfenidone had a significant inhibitory effect on HPF proliferation, migration and collagen synthesis. There were no differences between the cells treated with 0.5, 1.0 and 1.5 mg/mL pirfenidone and the controls in the MTT assay. After 48 h of treatment with 1.0 or 1.5 mg/mL pirfenidone, TGF-β expression was significantly decreased. CONCLUSIONS: These findings demonstrate that pirfenidone inhibits the proliferation, migration and procollagen secretion of HPFs at nontoxic concentrations by decreasing TGF-β expression. Thus, pirfenidone may be considered as a safe adjuvant for pterygium surgery to prevent recurrence.
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