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  • Title: [Both CK2 catalytic subunits involves in androgen receptor signaling in prostate cancer cells].
    Author: Yao K, Gao XY, Huang BJ, Li ZS, Zhou FJ, Li BY, Han H.
    Journal: Zhonghua Yi Xue Za Zhi; 2013 Nov 12; 93(42):3360-3. PubMed ID: 24418032.
    Abstract:
    OBJECTIVE: To explore the roles of different casein kinase 2 (CK2) catalytic subunits in androgen receptor (AR) signaling in prostate cancer cell lines. METHODS: Prostate cancer cell lines were maintained.Immunofluorescent staining was performed to determine AR nuclear translocation in PC-3/AR cells with R1881 pretreatment and luciferase gene reporter assay used to determine AR transactivation in LNCaP cells. And reverse transcription-polymerase chain reaction (RT-PCR) was employed to evaluate the mRNA level of prostate-specific antigen (PSA). RESULTS: R1881-induced AR nuclear localization was reduced significantly (P < 0.01).R1881-stimulated ARE-LUC reporter activity in LNCaP cells decreased with reduced level of PSA mRNA, an AR endogenous target (P < 0.05). To confirm the target specificity, the gene-specific siRNAs were used for both CK2α and CK2α' subunits. The results of ARE-LUC assay (38.5 vs 31.4) suggested that both CK2 catalytic subunits were involved in androgen-stimulated AR nuclear translocation and AR-mediated gene expression in prostate cancer cells. CONCLUSION: Both CK2 catalytic subunits are involved in androgen receptor signaling of prostate cancer cells.
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