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  • Title: [Isolation and immunochemical characteristics of soluble membrane proteins from the cell wall of Corynebacterium diphtheria].
    Author: Kondrashina NN, Shmeleva EA, Beresten' SF.
    Journal: Biokhimiia; 1987 Jun; 52(6):978-83. PubMed ID: 2444270.
    Abstract:
    A method for isolation of immunochemically active proteins from Corynebacterium diphtheria membranes was elaborated. The proteins were solubilized with the nonionic detergent NP-40 and gel-filtered through an Ultrogel AcA-34 column under denaturating conditions. The purified proteins (Mr = 64 kD) were antigenically active in a solid phase radioimmunoassay with human antidiphtheria antibodies.
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