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  • Title: A lateral flow assay for identification of Escherichia coli by ribosomal RNA hybridisation.
    Author: Pöhlmann C, Dieser I, Sprinzl M.
    Journal: Analyst; 2014 Mar 07; 139(5):1063-71. PubMed ID: 24443718.
    Abstract:
    Existing technologies for analysis of microbiological contaminants in food or clinical samples are often expensive and require laboratory settings and trained personnel. Here we present a lateral flow assay employing gold nanoparticle-oligodeoxynucleotide conjugates and four-component sandwich hybridisation for direct detection of specific sequences in bacterial 16S ribosomal RNA. Combined with rapid "one step" lysis the developed procedure allows detection of 5 × 10(4) colony forming units per mL Escherichia coli within less than 25 minutes. Several Escherichia coli strains were detected successfully, whereas non-related as well as closely related bacterial species produced no signal. The developed nucleic acid lateral flow assay is inexpensive, rapid to perform and requires no nucleic acid amplification step.
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