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  • Title: Streptavidin binding as a model to characterize thiol-ene chemistry-based polyamine surfaces for reversible photonic protein biosensing.
    Author: Melnik E, Muellner P, Bethge O, Bertagnolli E, Hainberger R, Laemmerhofer M.
    Journal: Chem Commun (Camb); 2014 Mar 07; 50(19):2424-7. PubMed ID: 24448367.
    Abstract:
    Biotin- and iminobiotin-bonded surfaces obtained by thiol-ene chemistry and subsequent modification with polyamines were characterized with respect to streptavidin-binding capacity and reversibility for photonic biosensing using X-ray photoelectron spectroscopy and Mach-Zehnder-interferometric sensors. The streptavidin-iminobiotin system was exploited for reversible multilayer deposition and determination of affinity constants on each layer.
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