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  • Title: Detection of a few picograms of DNA on polyacrylamide gels by silver staining.
    Author: Lomholt B, Frederiksen S.
    Journal: Anal Biochem; 1987 Jul; 164(1):146-9. PubMed ID: 2445219.
    Abstract:
    DNA fragments separated on polyacrylamide gels are silver stained in ethanolamine solution. The staining procedure can be completed in 3 1/2 h. Illumination of the gels on a black background increases the sensitivity of detection compared with the usual transillumination. The limit level of detection is 3-5 pg per band with a cross-sectional area of 5 mm2. Five to fifty picograms of DNA may be detected quantitatively by scanning the gels. The method will detect 0.1 to 1 ng per band of low-molecular-weight RNA components.
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