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  • Title: Preservation of resuspended red cell concentrate. Analysis of purines, nucleosides and nucleotides in stored red cells.
    Author: Walper A, Berndsen G, Strauss D.
    Journal: Folia Haematol Int Mag Klin Morphol Blutforsch; 1987; 114(4):463-4. PubMed ID: 2446973.
    Abstract:
    Purine nucleotides of red blood cells (RBC) during storage in two different media with addition of adenine/nicotinamide (NAP) or adenine/guanosine (CDS-AG) were estimated by HPLC. Synthesis of guanine nucleotides reached a maximum after 14 days in RBC stored with adenine/guanosine. The higher adenine concentration in the NAP solution (3 mmol/l) did not increase adenine consumption and the ATP-level of the erythrocytes. The adenylate energy charge (AEC) of RBC decreased from 0.91 to 0.63 during 42 days of storage in CDS-AG solution.
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