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  • Title: Antioxidants increase the cryoresistance of GM-CFC.
    Author: Matthes G, Hübner U, Gränz M, Schütt M, Hackensellner HA.
    Journal: Folia Haematol Int Mag Klin Morphol Blutforsch; 1987; 114(4):482-3. PubMed ID: 2446983.
    Abstract:
    The influence of antioxidative drugs on the cryopreservation of human bone marrow cells was studied. The viability of bone marrow cells was tested after freezing and thawing from--196 degrees C by the growth of GM-CFC in agar culture. The results suggest the ability of antioxidants to protect stem cells against damage caused by freezing and thawing. Addition of tocopherol or penicillin to a cryoprotective medium increases the number of surviving GM-CFC. From the colony-to-cluster ratio can be concluded that antioxidants especially protect more immature haematopoietic (colony forming) cells.
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