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  • Title: An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection.
    Author: Pitz Ade F, Koishi AC, Tavares ER, Andrade FG, Loth EA, Gandra RF, Venancio EJ.
    Journal: Rev Soc Bras Med Trop; 2013; 46(6):783-5. PubMed ID: 24474025.
    Abstract:
    INTRODUCTION: Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. METHODS: We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. RESULTS: The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or equivalent to the other nested or semi-nested PCR methods for P. brasiliensis detection. CONCLUSIONS: The OTsn-PCR assay described in this paper has a detection limit similar to other reactions for the molecular detection of P. brasiliensis, but this approach is faster and less prone to contamination than other conventional nested or semi-nested PCR assays.
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