These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: Identification of cDNA clones of the mouse neural cell adhesion molecule L1. Author: Tacke R, Moos M, Teplow DB, Früh K, Scherer H, Bach A, Schachner M. Journal: Neurosci Lett; 1987 Nov 10; 82(1):89-94. PubMed ID: 2447532. Abstract: Two cDNA clones of the neural cell adhesion molecule L1 (Mr 200,000) were isolated using lambda gt10 and lambda gt11 libraries constructed from postnatal day 8 mouse brain poly(A)+ RNA. Clone K21 was selected and identified using immunoaffinity purified polyclonal antibodies. It was then used to isolate a secondary clone (K21-1), which hybridized with an oligonucleotide probe synthesized by reverse translation of the aminoterminal sequence of the 80 kDa carboxyterminal proteolytic fragment of L1. Blot hybridization analysis indicated that L1 is encoded by a single gene and transcribed by a single 6 kb mRNA which is present only in cells or tissues known to express L1.[Abstract] [Full Text] [Related] [New Search]