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  • Title: Detection of carbapenemase activities of Bacteroides fragilis strains with matrix-assisted laser desorption ionization--time of flight mass spectrometry (MALDI-TOF MS).
    Author: Johansson A, Nagy E, Sóki J, ESGAI (ESCMID Study Group on Anaerobic Infections).
    Journal: Anaerobe; 2014 Apr; 26():49-52. PubMed ID: 24480431.
    Abstract:
    Today resistance against carbapenems is considered an emerging problem in Bacteroides fragilis. Carbapenemase activities produced by aerobic bacteria have been detected by looking at hydrolysis of carbapenems with MALDI-TOF MS, but this technique was never used for anaerobic bacteria. We have developed a protocol for detection and verification of carbapenemase production in B. fragilis within 2.5 h. Twenty-eight strains of B. fragilis were tested. Of the sixteen cfiA-positive strains all showed hydrolysis of ertapenem, whereas the twelve cfiA-negative strains showed no hydrolysis. Ertapenem hydrolysis could be inhibited with 2,6-Pyridinecarboxylic acid (DPA) in all cfiA-positive strains, verifying the presence of the metallo-beta-lactamase. Here we show a rapid way to detect carbapenemase activities of B. fragilis strains.
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