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  • Title: A simple ELISA for the classification of monoclonal antibodies according to their recognition of native epitopes.
    Author: Kenett D.
    Journal: J Immunol Methods; 1988 Feb 10; 106(2):203-9. PubMed ID: 2448386.
    Abstract:
    A sandwich ELISA for testing whether pairs of monoclonal antibodies recognize the same native antigenic site was developed. The assay was performed with murine anti-lysozyme monoclonal antibodies. A monoclonal antibody, adsorbed onto a microtiter plate, was used as a capture antibody for native lysozyme. After the reaction with the antigen a second monoclonal antibody, the test antibody, was added. The amount of bound antibody was quantitatively measured using rabbit anti-mouse immunoglobulin serum conjugated to alkaline phosphatase. The simultaneous binding of pairs of monoclonal antibodies to lysozyme was further substantiated by structural considerations.
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