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  • Title: [Functional analysis of VicK kinase activity in Streptococcus mutans].
    Author: Zhang Q, Wang SD, Zhou XD, Cheng L, Li YQ, Senadheera DB, Cvitkovitch DG.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2013 Nov; 44(6):911-5. PubMed ID: 24490500.
    Abstract:
    OBJECTIVE: To investigate the regulatory function on physiology and virulence of VicK kinase activity in Streptococcus mutans. METHODS: PCR ligation mutagenesis was used to construct a vicK knock-out mutant, and kinase activity abolished VicK was expressed by a streptococcal vector in this vicK null mutant. Colony morphology, overnight culture, biofilm formation and gene expression involved in biofilm formation were analyzed. Delta VicK, strains harboring a complemented wild-type vicK, and a vector without insert were used as controls. RESULTS: Colonies of VicK(H217A) were smoother and more elevated than that of wild-type UA159 and complementary strain SMCVicK; cells from VicK(H217A) overnight culture coaggregated on the bottom of glass tubes; no obvious alteration was observed in VicK(H217A) biofilm; expressions of gbpB, ftf, gtfD were repressed while gtfB/C were up-regulated (P < 0.05). CONCLUSION: VicK kinase activity is important for maintaining normal growth, biofilm formation and expression of genes involved in biofilm formation in Streptococcus mutans.
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