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  • Title: Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry.
    Author: Yang M, Wu W, Ruan Y, Huang L, Wu Z, Cai Y, Fu F.
    Journal: Anal Chim Acta; 2014 Feb 17; 812():12-7. PubMed ID: 24491758.
    Abstract:
    In this study, an ultra-sensitive method for the quantification of lysozyme based on the Gd(3+) diethylenetriamine-N,N,N',N″,N″-pentaacetic acid labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) was described. The Gd(3+)-tagged lysozyme was effectively separated by capillary electrophoresis (CE) and sensitively determined by inductively coupled plasma mass spectrometry (ICP-MS). Based on the gadolinium-tagging and CE-ICP-MS, the lysozyme was determined within 12 min with an extremely low detection limit of 3.89 attomole (3.89×10(-11) mol L(-1) for 100 nL of sample injection) and a RSD<6% (n=5). The proposed method has been successfully used to detect lysozyme in saliva samples with a recovery of 91-106%, suggesting that our method is sensitive and reliable. The success of the present method provides a new potential for the biological assays and sensitive detection of low-abundant proteins.
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