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  • Title: Monoclonal antibodies and characterization of epitopes of smooth Brucella lipopolysaccharides.
    Author: Greiser-Wilke I, Moennig V.
    Journal: Ann Inst Pasteur Microbiol; 1987; 138(5):549-60. PubMed ID: 2449902.
    Abstract:
    Four mouse monoclonal antibodies generated against Brucella melitensis 16M, and three generated against B. suis 1330 were analysed. An ELISA (enzyme-linked-immunosorbent assay) with whole cells as antigens was used to determine cross-reactivities with other Gram-negative bacteria. Two antibodies showed cross-reactivity with smooth Brucella strains only. Five antibodies also reacted with Yersinia enterocolitica O:9, but not with other bacteria. Two of these antibodies had significantly higher titres with A greater than M serotype Brucella strains, indicating that these epitopes are related to the antigenic A complex. The antigenic determinants recognized by the monoclonal antibodies showed varying degrees of susceptibility towards oxidation. They were shown by immunoblotting to be located on the polysaccharide moiety of the O-side chain.
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