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  • Title: Scavenger receptor-mediated endocytosis facilitates RNA interference in the desert locust, Schistocerca gregaria.
    Author: Wynant N, Santos D, Van Wielendaele P, Vanden Broeck J.
    Journal: Insect Mol Biol; 2014 Jun; 23(3):320-9. PubMed ID: 24528536.
    Abstract:
    RNA interference (RNAi) has become a widely used loss-of-function tool in eukaryotes; however, the delivery of double-stranded (ds)RNA) to the target cells remains a major challenge when exploiting the RNAi-technology. In insects, the efficiency of RNAi is highly species-dependent. Yet, the mechanism of cell entry in insects has only been characterized in a cell line of the fruit fly, Drosophila melanogaster, a species that is well known to be poorly amenable to environmental RNAi. In the present paper, we demonstrate that silencing vacuolar H-ATPase 16 (vha16) and clathrin heavy chain (clath), two components of the Clathrin-dependent endocytosis pathway, together with pharmacological inhibition of scavenger receptors with polyinosine and dextran sulphate, can significantly attenuate the highly robust RNAi response in the desert locust, Schistocerca gregaria.
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