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  • Title: Voltage-dependent pore activity of the peptide alamethicin correlated with incorporation in the membrane: salt and cholesterol effects.
    Author: Stankowski S, Schwarz UD, Schwarz G.
    Journal: Biochim Biophys Acta; 1988 Jun 07; 941(1):11-8. PubMed ID: 2453215.
    Abstract:
    Strong aggregation of incorporated alamethicin in the bilayer of lipid vesicles has been observed spectroscopically at aqueous peptide concentrations above a critical value c*. On the other hand, in conventional gating studies with planar lipid films, the onset of conducting pore formation can be characterized by a threshold voltage V.. We present experimental evidence of a direct correspondence between the effects on c* and V. when these parameters are modulated by adding NaCl (to the aqueous medium) or cholesterol (to the lipid moiety). A quantitative analysis supports the idea that the measured aggregation actually results in pore formation, the voltage-dependence being due to an electric field effect on the partition equilibrium of the peptide between the aqueous and the lipid phases.
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