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  • Title: Isolation of HIV from Israeli patients and asymptomatic carriers.
    Author: Vonsover A, Gotlieb-Stematsky T, Alexander R, Fogel A, Rubinstein E, Handzel ZT, Burstein R, Bentwich Z.
    Journal: Isr J Med Sci; 1988 Mar; 24(3):141-4. PubMed ID: 2453484.
    Abstract:
    Human immunodeficiency virus (HIV 1) strains were isolated from peripheral blood lymphocytes of eight homosexual men (two asymptomatic and six with lymphadenopathy syndrome) and of the wife of an AIDS patient with AIDS-related complex. All except one had antibodies to HIV in their serum. Virus isolation was carried out by cocultivation of the donors' peripheral lymphocytes with HUT-78 cells or with phytohemagglutinin-stimulated normal cord blood lymphocytes. Identification of the virus was based on detection of specific cytopathogenic effects, determination of reverse transcriptase activity, specific immunofluorescence and Southern blot analysis of HIV proviral DNA in the infected cell cultures. The 1st isolation of HIV in Israel is recorded in the report. Out of 11 homosexual men with antibodies to HIV, the virus was found in 7 of the men. In addition, the virus was found in a seropositive woman with ARC who was the wife of a bisexual patient. The isolation of HIV appeared more efficient in ARC or LAS patients. Isolation of HIV is done through cocultivation of the patient's peripheral lymphocytes with HUT-78 cells or with phytohemagglutinin-stimulated normal cord blood lymphocytes. Because of the unique characteristics of HIV, a reverse transcriptase activity, cytopathic effects on target T-lymphocyte and syncytium of giant and multinucleated cells, identification of the virus was based on the detection of these qualities. HIV antibodies located by immunoassays and immunofluorescence. The virus was isolated in 50% of the AIDS patients, 85% of ARC patients and 60% of LAS patients. Knowing the characters of currently isolated strains of HIV will help researchers to understand the initial stages and movement of the disease through Israel and help them develop steps towards its control.
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