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  • Title: Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method.
    Author: Lin WC, Liu WL, Tang WY, Huang CP, Huang HY, Chin TY.
    Journal: Electrophoresis; 2014 Jun; 35(11):1751-5. PubMed ID: 24535662.
    Abstract:
    In this study, detection of 20 FITC-derivatized amino acids using an MEEKC-LIF was demonstrated. In order to achieve good separation for hydrophobic amino acids, the MEEKC method was employed and detection limits were obtained in the range of 0.32-2.2 nM, which is comparable to previous reports on amino acid analyses. Furthermore, a significant reduction in the reaction time from 1 h for conventional derivatization to 3 min for the microwave-assisted derivatization was observed and achieved, as opposed to the traditional pretreatment of real sample due to its complexity prior to the analysis of amino acid content. Finally, this microwave-assisted derivatization MEEKC-LIF method successfully determined amino acids in beverage, food, and biological samples (rat brain) with good recovery.
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