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  • Title: Studies on the clonality of the response to an epitope of a protein antigen. Randomness of activation of epitope-recognizing clones and the development of clonal dominance.
    Author: Benjamini E, Andria ML, Estin CD, Norton FL, Leung CY.
    Journal: J Immunol; 1988 Jul 01; 141(1):55-63. PubMed ID: 2454264.
    Abstract:
    Syngeneic mice immunized with tobacco mosaic virus protein (TMVP) can differ with respect to their ability to produce antibodies that bind a decapeptide epitope representing residues 103 to 112 of TMVP, and with respect to the fine specificity of the decapeptide binding antibodies as determined by their ability to bind several synthetic analogues of the decapeptide. To elucidate the mechanism responsible for the differences between the syngeneic animals in their ability to make anti-decapeptide antibodies, spleen cells from a large number of naive CSW mice were pooled, and aliquots were transferred (either including or excluding resident T cells) into naive recipients that were subsequently immunized with TMVP. Examination of the frequency and fine specificity of anti-decapeptide antibodies revealed that the recipients exhibited various clonalities of decapeptide binding antibody responses similar to those seen in a normal population of CSW mice. Moreover, the response of each individual mouse was of a restricted clonality despite the availability of a more extensive repertoire of decapeptide-recognizing clones. The results indicate that the selection of the clonality of the antibody response was not determined by the presence (or absence) of particular clones of B or T cells and that the mechanism responsible for generating differences between mice must have acted, subsequent to introduction of the Ag, by activation of a limited number of clones randomly selected by Ag and/or by Ag-driven mutation. The long term nature of the antibody response to the decapeptide epitope was also investigated. The response was shown to be "locked-in" for the life of the immunized individual. Thus, individuals that responded to TMVP but that did not produce antibodies to the decapeptide after the first set of immunizations with TMVP maintained their non-responsiveness to the decapeptide after the second set of immunizations with the protein. However, individuals that responded to an initial set of immunizations with TMVP by producing antibodies to the decapeptide epitope continue to produce antibodies to the decapeptide after a second set of immunizations with TMVP. The fine specificity of the decapeptide-binding antibodies also appeared to be "locked in" throughout the life of the immunized individual. The long term maintenance of the clonability of the antibody response does not appear to be influenced by Ag-specific T cells and is strictly a function of memory B cells.(ABSTRACT TRUNCATED AT 400 WORDS)
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