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  • Title: Identification of soluble supramolecular FN-fibrin complexes in human plasma.
    Author: Krzyżanowska-Gołąb D, Lemańska-Perek A, Pupek M, Lindner K, Polańska B, Porębska I, Kątnik-Prastowska I.
    Journal: J Immunoassay Immunochem; 2014; 35(4):412-27. PubMed ID: 24547768.
    Abstract:
    SDS-agarose FN immunoblotting of 257 normal and pathological human plasma samples revealed the ladder pattern of multiple plasma FN bands which corresponded to FN monomer and dimer, and 5 FN-fibrin bands with increasing molecular masses. The FN-fibrin bands of about 750 kDa, 1000 kDa, 1300 kDa, 1600 kDa, and 1900 kDa appeared more frequently and in significantly higher relative amounts in the pathological samples (P < 0.000) than in relatively healthy individuals. The revealing of high-molecular FN-fibrin complexes by SDS-agarose FN immunobloting might have the potential to become a laboratory biomarker of some diseases in which the coagulation system is triggered.
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