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  • Title: Improved avidin-biotin-peroxidase complex (ABC) staining.
    Author: Cattoretti G, Berti E, Schiró R, D'Amato L, Valeggio C, Rilke F.
    Journal: Histochem J; 1988 Feb; 20(2):75-80. PubMed ID: 2455699.
    Abstract:
    A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the immunostained site and allowed the subsequent binding of a complementary complex. With this technique a higher dilution of the antisera could be used and minute amounts of antigen masked by the fixative could be demonstrated on paraffin sections.
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