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  • Title: Fluorography of tritium-labeled proteins in silver-stained polyacrylamide gels.
    Author: Kulcsár P, Prestwich GD.
    Journal: Anal Biochem; 1988 May 01; 170(2):528-31. PubMed ID: 2456027.
    Abstract:
    Silver-staining of polyacrylamide gel electrophoresis (PAGE)-separated proteins allows sensitive detection of proteins but severely reduces the ability to detect weak beta-emitters present in the protein band. A simple procedure is described in which silver can be removed from a silver-stained PAGE gel (deargentation) using photographic fixer, and the silver-free gel can be enhanced and used for fluorography. A quantitative study of sensitivity is reported for 3H-labeled bovine serum albumin with a one-dimensional sodium dodecyl sulfate-PAGE slab gel.
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