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  • Title: Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of Portugal by ELISA.
    Author: Silva CP, Lima DL, Schneider RJ, Otero M, Esteves VI.
    Journal: Sci Total Environ; 2014 May 01; 479-480():227-32. PubMed ID: 24561298.
    Abstract:
    This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1-100 μg L(-1). Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between <LOD and 15 μg L(-1). Results correlate well with those obtained by LC-MS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters.
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