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  • Title: Neisseria gonorrhoeae recombinant strains expressing hybrid serological reactivities of outer membrane proteins IA and IB.
    Author: Shinners EN, Catlin BW.
    Journal: J Infect Dis; 1988 Sep; 158(3):529-36. PubMed ID: 2457634.
    Abstract:
    The inheritance of epitopes of protein I, the principal protein of the outer membrane of Neisseria gonorrhoeae, was investigated by DNA-mediated transformation. Protein I transformants were isolated by selection for the linked spectinomycin-resistance determinant. Twelve monoclonal antibodies used in coagglutination tests identified epitopes of the two forms of protein I (P.IA and P.IB). A given gonococcal culture from patients expresses epitopes of either P.IA or P.IB and rarely, if ever, exhibits hybrid P.IA/P.IB reactivities. Nevertheless, we found 35 P.IA/P.IB recombinants among 1506 transformants. Transmission by DNA of the hybrid reactivities and the apparent molecular mass characteristic of a given P.IA/P.IB species verified the genetic basis of the phenotypic changes. A recombinant that expressed six P.IA and two P.IB epitopes is of interest as a possible component of a gonococcal vaccine, because one or more of these epitopes are shared with 99.8% of a worldwide collection of 1858 clinical strains.
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