These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A surface-enhanced Raman scattering method for detection of trace glutathione on the basis of immobilized silver nanoparticles and crystal violet probe.
    Author: Ouyang L, Zhu L, Jiang J, Tang H.
    Journal: Anal Chim Acta; 2014 Mar 13; 816():41-9. PubMed ID: 24580853.
    Abstract:
    Unsatisfactory sensitivity and stability for molecules with low polarizability is still a problem limiting the practical applications of surface-enhanced Raman scattering (SERS) technique. By preparing immobilized silver nanoparticles (Fe3O4/Ag) through depositing silver on the surface of magnetite particles, a highly sensitive and selective SERS method for the detection of trace glutathione (GSH) was proposed on the basis of a system of Fe3O4/Ag nanoparticles and crystal violet (CV), in which the target GSH competed with the CV probe for the adsorption on the Fe3O4/Ag nanoparticles. Raman insensitive GSH replaced the highly Raman sensitive CV adsorbed on the surface of Fe3O4/Ag particles. This replacement led to a strong decrease of the CV SERS signal, which was used to determine the concentration of GSH. Under optimal conditions, a linear response was established between the intensity decrease of the CV SERS signal and the GSH concentration in the range of 50-700 nmol L(-1) with a detection limit of 40 nmol L(-1). The use of a Fe3O4/Ag substrate provided not only a great SERS enhancement but also a good stability, which guarantees the reproducibility of the proposed method. Its use for the determination of GSH in practical blood samples and cell extract yielded satisfactory results.
    [Abstract] [Full Text] [Related] [New Search]