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  • Title: High-throughput cloning for biophysical applications.
    Author: Kwon K, Peterson SN.
    Journal: Methods Mol Biol; 2014; 1140():61-74. PubMed ID: 24590709.
    Abstract:
    Efficient high-throughput gene cloning represents a critical first step for conducting functional and structural proteomics in the post-genomic era. The ligation-independent cloning (LIC) method has been almost universally adopted by large structural biology centers as a component of high-throughput structure determination pipelines. The LIC platform is easy to use, of low cost, and rapid, and importantly, it is easily adapted to 96- or 384-well format, thereby facilitating automation. Procedures are described for 96-well format cloning using the LIC technology.
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